In the depth cooperation between school and enterprise, this paper puts forward the training innovation mode for C language course, getting rid of the traditional mode of "knowledge + case". It changes the practical teaching of C language course to be project oriented, specialized and professional. It takes the real business case as the carrier to guide the students to complete the project development process step by step. The professional knowledge of students has been consolidated, their professional skills have been improved, and their project execution, occupation skills and literacy also have been effectively trained.