Microprocessor Principle and Application is a theoretical and practical course, which has both hardware and software design knowledge. In the course teaching, adopting the traditional teaching method is difficult to achieve the purpose of innovative talents training. Aiming at the problems, with the education concept of cultivating innovative talents for universities of applied sciences, and according to the course content and characteristics, the thesis puts forward the teaching reform from four aspects including simulation teaching, practical teaching, project-driven teaching and examination reform.