In the production of the film, color-mixing is an essential link. In the actual production process, the film is required to turn into a certain color, or the scenery is adjusted reasonable and coordinate. Some links has high requirements in color-mixing, especially the color of characters. Based on the basic knowledge of color, this paper analyzes on the related knowledge of color-mixing using After Effects, which will achieve twice the result with half the effort to apply After Effects in the color-mixing and film production.