This paper analyzes the demand of large instrument and equipment sharing platform construction and the characteristics of the sharing platform. Based on the three-layer structures of B/S, the paper uses a modular design concept, develops the platform with ASP.NET and C# based on Microsoft.NET platform. Internet-based resource sharing system has advantages of resources opening up, information updating quickly, simple management and convenient operation. It solves the problems of Resource Island and difficultly sharing, and offers an effective way for the scientific management and sharing of large-scale instruments in university.