Abstract:The ISA bus is industrial standard bus architecture,which survives a long time in the industrial area,is the first system-level bus.USB bus is more and more popular because of the convenient features that make its application extensively. USB interface equipments become more and more in the electronic market,USB bus replaces the traditional old-fashioned interface will become a trend.The paper introduces the USB bus and the ISA bus;then mainly introduces the design principle, hardware design and software design of the transfer cards based on CH375 chip;finally introduces the applications of adapter card in engineering.